Cov nyhuv ntawm pH tus nqi thiab qhov kub thiab txias ntawm pyrrolidone ntawm PVP aqueous tov plays lub luag haujlwm hauv kev khaws cov noob ntawm tes. Kev ua haujlwm kom raug thiab tsim nyog khov kho tuaj yeem txo qhov kev hloov pauv lossis poob ntawm cov yam ntxwv ntawm tes. Uncross-linked PVP tov tsis muaj tshwj xeeb thixotropy, tshwj tsis yog tias nws yog thixotropic ntawm siab concentration thiab qhia tau hais tias lub sij hawm so luv heev. Kev paub txog kev ua kom khov ntawm tes: Xaiv lub cuab yeej khov kho yog qhov zoo tshaj plaws. Cell khov yog ib qho cuab yeej uas khaws cov cell hauv qhov chaw tsis muaj qhov kub thiab txias, txo cov metabolism hauv cell, thiab ua tiav cov khoom cia ntev. (Pyrrolidone) Nyob rau hauv feem ntau cov kab ntawm tes, cov thickening kev ua tau zoo ntawm PVP yog ze ze rau nws cov txheeb ze molecular loj. Nyob rau hauv cov ntsiab lus concentration, qhov loj dua qhov txheeb ze molecular mass, qhov ntau dua nws cov viscosity. Cells yuav txuas ntxiv mus ntxiv thiab hloov nrog kev laus thiab kev hloov pauv, ua rau "kev coj noj coj ua" ntawm phenotype thiab genotype. Kev kho kom raug thiab ua tiav kev khov kho ua lub luag haujlwm tseem ceeb hauv kev siv lub sijhawm ntev ntawm cov hlwb.
Thaum lub sij hawm khov, yog li ntawd, cov kab lis kev cai raug thiab maj mam sau ntawm tes, ua ntej khov, cov hlwb yuav tsum tswj hwm txoj kev loj hlob zoo tshaj plaws (hauv logarithmic lossis exponential theem). Qhov zoo tshaj plaws, kab lis kev cai nruab nrab yuav tsum tau hloov 24 teev ua ntej sau qoob. Nws raug nquahu kom kuaj xyuas kab lis kev cai rau cov kab mob microbial, tshwj xeeb tshaj yog mycoplasma, kom ntseeg tau tias cov hlwb tsis muaj kab mob. Thaum lub sijhawm sau cov txheej txheem ntawm tes, kev sim ua haujlwm yuav tsum maj mam li sai tau kom tsis txhob muaj kev puas tsuaj ntawm tes. (Pyrrolidone) Tsim nyog cryoprotectant. Tam sim no, cov cuab yeej siv feem ntau rau kev ua kom khov ntawm tes yog kua nitrogen cryopreservation, uas feem ntau yog siv txoj kev khov qeeb nrog tus nqi tsim nyog ntawm tus neeg saib xyuas kom khov rau cov hlwb thiab txo cov kev puas tsuaj rau cov hlwb thaum lub sij hawm khov. Yog tias cov cell ncaj qha khov yam tsis muaj tus neeg tiv thaiv, cov dej sab hauv thiab sab nraud ntawm lub hlwb yuav ua rau cov dej khov sai sai, ua rau muaj kev cuam tshuam tsis zoo.
Polyvinyl pyrrolidone, ethylene glycol, methanol thiab methylacetamide yog txhua yam cryoprotectants. Dimethyl sulfoxide (DMSO) thiab glycerol yog ib txwm muaj nyob rau hauv cellopreservation. Ob yam khoom no muaj qhov hnyav molecular, siab solubility, thiab tuaj yeem nkag mus tau yooj yim ntawm cov hlwb. Lawv tuaj yeem txo qhov chaw khov thiab nce permeability ntawm cell membranes rau dej. Qhov tseem ceeb yog nyob rau hauv lub cell xwm. (Pyrrolidone) DMSO feem ntau muaj qhov concentration ntawm 5-10% (v/v), thiab qhov pom kev zoo sib txawv nrog cov kab sib txawv. Qhov kawg concentration ntawm glycerol nyob rau hauv lub freezing nruab nrab yog 5-15%. Ib yam li ntawd, qhov zoo tshaj plaws concentration nyob ntawm cov kab ntawm tes. Txhawm rau kom muaj sia nyob ntawm cov hlwb uas nyuaj rau khaws cia, koj tuaj yeem xaiv kom nce cov ntshav concentration hauv cov tshuaj khaws cia thaum lub sij hawm cryopreservation. Yog tias koj xav kom khov cov hlwb sai dua thiab tsis tu ncua, kev daws teeb meem ntawm tes yuav yog qhov kev xaiv zoo. Tsis tas yuav tsum tau npaj. Tsuas yog ntxiv qhov tsim nyog ntawm cov tshuaj khov rau cov hlwb. Kev ua haujlwm yog yooj yim thiab koj tuaj yeem muaj cov hlwb nrog kev ua haujlwm siab.
